一种交联壳聚糖/透明质酸复合多孔支架的制备方法

Preparation method of crosslinked chitosan/hyaluronic acid compound porous scaffold

Abstract

The invention relates to the technical field of new materials and relates to a preparation method of a crosslinked chitosan/hyaluronic acid compound porous scaffold. According to the preparation method, natural biological materials such as chitosan and hyaluronic acid are used as raw materials and an organic silane coupling agent KH-560 is used as a crosslinking agent, and the crosslinked chitosan/hyaluronic acid compound porous scaffold which is made of purely natural biological materials and is excellent in mechanical property is prepared adopting a freeze-drying method. The crosslinked chitosan/hyaluronic acid compound porous scaffold prepared by adopting the preparation method has the characteristics of high porosity, high pores strength, large pores diameter and the like, is of an open type pore structure that small pores are contained in large pores, and is suitable for planting cells on the surface of the scaffold, wherein the pore diameter is 100-300 mu m and porosity is more than 90%. Compared with a chitosan scaffold, the crosslinked chitosan/hyaluronic acid compound porous scaffold has the advantages that the mechanical property is better; proliferation and differentiation effect of the cells on the surface of the porous scaffold is better due to the addition of the organic silane coupling agent, and affinity to biological cells is better.
本发明属新材料技术领域,具体涉及一种交联壳聚糖/透明质酸复合多孔支架的制备方法。该方法是以壳聚糖和透明质酸等天然生物材料为原料,有机硅烷偶联剂KH-560为交联剂,采用冷冻干燥法,制备出力学性能优良的由纯天然生物材料组成的交联壳聚糖/透明质酸复合多孔支架。本发明制备的交联壳聚糖/透明质酸复合多孔支架具有高孔隙率、高孔强度和大孔径等特点,其孔径为100~300µm,孔隙率>90%,为大孔含小孔的开放型孔结构,适合在支架表面种植细胞。相比壳聚糖支架,该交联壳聚糖/透明质酸复合多孔支架具有更好的力学性能,透明质酸和有机硅交联剂的加入,更有利于细胞在多孔支架表面增殖和分化,对生物细胞的亲和性更好。

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Cited By (1)

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    CN-104874017-ASeptember 02, 2015中国人民解放军总医院复合Tβ4的胶原-壳聚糖组织工程支架及其制备方法和应用