串珠镰刀菌素胶体金试纸条的制备及使用方法

Preparation and application method of fusarium moniliforme colloidal gold test strip

Abstract

本发明属生物检测领域,涉及一种串珠镰刀菌素胶体金试纸条的制备及使用方法,其特征在于:包括纸板,纸板的一面从上到下依次粘贴吸水垫、检测垫、金标垫和样品垫,相邻各垫在连接处交叠连接,所述检测垫以硝酸纤维素膜为基垫,硝酸纤维素膜上自上而下设置横向参考线和检测线,所述检测线包被有串珠镰刀菌素-牛血清白蛋白偶联物,参考线包被有兔抗鼠多克隆抗体;所述金标垫横向喷涂有纳米金标记的抗串珠镰刀菌素单克隆抗体,所述抗串珠镰刀菌素单克隆抗体由杂交瘤细胞株产生。该试纸条用于检测串珠镰刀菌素,具有检测快速、操作简单、灵敏度高的特点。
The invention belongs to the field of biological detection, and relates to a preparation and application method of a fusarium moniliforme colloidal gold test strip. The fusarium moniliforme colloidal gold test strip is characterized by comprising a paperboard, wherein a water absorption pad, a detection pad, a gold mark pad and a sample pad are pasted on one side of the paperboard sequentially from top to bottom; the adjacent pads are overlapped and connected at the joint; in the detection pad, a nitrocellulose membrane is used as a base pad; the nitrocellulose membrane is provided with a transverse reference line and a detection line from top to bottom; the detection line coats a fusarium moniliforme-bovine serum albumin conjugate, and the reference line coats a rabbit antimouse polyclonal antibody; the gold mark pad is transversely sprayed with a nanogold-marked monoclonal antibody against fusarium moniliforme, which is generated by a hybridoma cell strain. The test strip is used for detecting the fusarium moniliforme and has the characteristics of quick detection, simplicity in operation and high sensitivity.

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